y984  (Cell Signaling Technology Inc)

Journal: Food Science & Nutrition

Article Title: Isothiocyanates attenuate heparin‐induced proliferation of colon cancer cells in vitro

doi: 10.1002/fsn3.4296

Figure Lengend Snippet: (a) Illustrative images depicting the expression levels of ErbB1, ErbB2, ErbB4, and β ‐actin. (b) Levels of ErbB1, ErbB2, and ErbB4 normalized to β ‐actin. (c) Gene expression levels of ErbB1, ErbB2, and ErbB4 measured by qPCR. Data are presented as mean ± SD ( n ≥ 3). # p < .1, ## p < .1, and #### p < .0001 compared with the control group, * p < .1, ** p < .01, *** p < .001, and **** p < .0001 compared with the heparin group.

Article Snippet: Protein bands were then transferred to polyvinylidene fluoride (PVDF) membranes (Millipore, USA) and blocked with 5% nonfat milk in TBST (Tris‐buffered saline containing 0.1% Tween 20) at room temperature for 2 h. The membranes were incubated overnight with primary antibodies at 4°C targeting TGF‐ β (CST, 3709S), EGFR (ErbB1) (epidermal growth factor receptor) (CST, 4267T), HER2 (ErbB2) (receptor tyrosine‐protein kinase erbB‐2) (CST, 4290T), HER3 (ErbB3) (receptor tyrosine‐protein kinase erbB‐3) (CST, 12708T), HER4 (ErbB4) (receptor tyrosine‐protein kinase erbB‐4) (CST, 4795T), Bcl‐2 (B‐cell lymphoma 2) (CST, 3498T), Bax (Bcl‐2‐associated X protein) (CST, 2772T), and β ‐actin (CST, 8457T).

Techniques: Expressing, Gene Expression, Control

Journal: Analytical sciences : the international journal of the Japan Society for Analytical Chemistry

Article Title: Mechanistic investigation into selective cytotoxic activities of gold nanoparticles functionalized with epidermal growth factor variants.

doi: 10.1007/s44211-022-00256-7

Figure Lengend Snippet: Fig. 5 Analysis of the activation state of the EGFR family in A431 cells treated with various EGF-GNPs and GNPs without EGF by EGFR phosphoryla- tion array. Cells were treated with 6 nM of (A) WT-GNPs, (B) SR-GNPs, (C) RS-GNPs, and (D) GNPs without EGF for 10 min. (E) The map of the human EGFR phosphorylation array used in this assay. P1, P2, P3, P4: positive control spots. Neg: negative spots. EGFR, ErB2, ErB3, ErB4: the meas- ured expression level of EGFR family. Y845, Y992, Y1045, Y1068, Y1086, Y1148, Y1173, S1046/1047: phosphorylation sites in EGFR. Y877, Y1112, Y1221/1222, Y1248, T686, S1113: phosphorylation sites in HER2. Y1289: a phospho- rylation site in ErB3. Y1284: a phosphorylation site in ErB4

Article Snippet: The antibodies for EGFR (D38B1) and phospho-EGFR at different phosphorylation sites (Y1068, Y845, and Y1173) were obtained from Cell Signaling Technology (MA, USA).

Techniques: Activation Assay, Phospho-proteomics, Positive Control, Expressing